Journal: STAR Protocols

Article Title: Protocol for evaluating CD8 + T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

doi: 10.1016/j.xpro.2022.101479

Figure Lengend Snippet: Viral RNA from chronically-infected monkeys treated with LP-98 monotherapy Plasma viral RNA was detected by TaqMan RT-PCR. Figure reprinted with permission from Xue et al. (2022) . (A) Plasma viral RNA of SVR monkeys with LP-98 monotherapy. (B) Plasma viral RNA of SVC monkeys with LP-98 monotherapy.

Article Snippet: TaqMan® RNA-to-C T TM 1-Step Kit , AB Applied Biosystems , CAT#: 4392938.

Techniques: Infection, Reverse Transcription Polymerase Chain Reaction

Journal: STAR Protocols

Article Title: Protocol for evaluating CD8 + T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

doi: 10.1016/j.xpro.2022.101479

Figure Lengend Snippet: Depletion of CD8 + T cells and viral rebound for SVC monkeys CD8 + T cell counts and plasma viral RNA were monitored by flow cytometry and TaqMan RT-PCR separately after injecting depleting anti-CD8 antibody into SVC monkeys. Shaded areas are displayed as the SD. Figure reprinted with permission from Xue et al. (2022) . (A) CD8 + T cell counts of SVC monkeys following anti-CD8 antibody adoptive infusion. (B) Plasma viral RNA of SVC monkeys following anti-CD8 antibody adoptive infusion.

Article Snippet: TaqMan® RNA-to-C T TM 1-Step Kit , AB Applied Biosystems , CAT#: 4392938.

Techniques: Flow Cytometry, Reverse Transcription Polymerase Chain Reaction

Journal: STAR Protocols

Article Title: Protocol for evaluating CD8 + T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

doi: 10.1016/j.xpro.2022.101479

Figure Lengend Snippet:

Article Snippet: TaqMan® RNA-to-C T TM 1-Step Kit , AB Applied Biosystems , CAT#: 4392938.

Techniques: Recombinant, Staining, Cell Stimulation, Software, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: PLoS ONE

Article Title: Severe Blunt Muscle Trauma in Rats: Only Marginal Hypoxia in the Injured Area

doi: 10.1371/journal.pone.0111151

Figure Lengend Snippet: Animals were ventilated with 100% O 2 . The traumatized Musculus gastrocnemius of the trauma group IIa (n = 4 for each time point) and the Musculus gastrocnemius of the control group IIc (n = 6) were harvested and sections of the muscle specimen were analyzed for pimonidazole binding. Hypoxic fraction, percentage area of pimonidazole-labelled muscle cells within an area; injured area, injured area of the traumatized muscle of the trauma group IIa; uninjured area, the adjacent uninjured area of the traumatized muscle of the trauma group IIa; control, tissue area of the non-traumatized muscle of the control group IIc. Values shown represent means ± SEM. *P<0.05 (versus control).

Article Snippet: Immunohistochemical detection of covalently bound pimonidazole was performed according to the manufacturer's protocol for pimonidazole staining (Hypoxyprobe- TM 1-Kit) with several modifications.

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Severe Blunt Muscle Trauma in Rats: Only Marginal Hypoxia in the Injured Area

doi: 10.1371/journal.pone.0111151

Figure Lengend Snippet: The animal was ventilated with 100% O 2 . 450 minutes after trauma, the Musculus gastrocnemius of the traumatized right hind limb was harvested and section of the muscle specimen was analyzed for pimonidazole labelling within the injured area. Injured area is identified by e.g. necrotic muscle cells (arrow) and edema (arrowhead). Hypoxic muscle cells indicated by pimonidazole binding are stained brown (star). Scale bar: 500 µm.

Article Snippet: Immunohistochemical detection of covalently bound pimonidazole was performed according to the manufacturer's protocol for pimonidazole staining (Hypoxyprobe- TM 1-Kit) with several modifications.

Techniques: Binding Assay, Staining